Paucibacterial Microbiome and Resident DNA Virome of the Healthy Conjunctiva.

ABSTRACT:

Purpose

To characterize the ocular surface microbiome of healthy volunteers using a combination of microbial culture and high-throughput DNA sequencing techniques.

Methods

Conjunctival swab samples from 107 healthy volunteers were analyzed by bacterial culture, 16S rDNA gene deep sequencing (n = 89), and biome representational in silico karyotyping (BRiSK; n = 80). Swab samples of the facial skin (n = 42), buccal mucosa (n = 50), and environmental controls (n = 27) were processed in parallel. 16S rDNA gene quantitative PCR was used to calculate the bacterial load in each site. Bacteria were characterized by site using principal coordinate analysis of metagenomics data. BRiSK data were analyzed for presence of fungi and viruses.

Results

Corynebacteria, Propionibacteria

SUBMITTER: Doan T 

PROVIDER: S-EPMC5054734 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature