Gene expression changes in HLA mismatched mixed lymphocyte cultures reveal genes associated with allorecognition.

ABSTRACT: Human leucocyte antigen (HLA) compatibility is the main factor determining the occurrence of graft-vs-host disease (GVHD) in patients. It has also been shown that minor histocompatibility antigen differences as well as genetic polymorphisms that are not sequenced by standard methodology for HLA typing can play a role. We used mixed lymphocyte cultures (MLCs) as a functional cellular test and investigated gene expression changes driven by HLA incompatibility in an effort to better understand the mechanisms involved in the disease. Gene expression profile of HLA matched and HLA mismatched MLC identified differentially regulated genes and pathways. We found that a great number of genes related to immune function were differentially regulated; these genes were also found to be associated with

SUBMITTER: Nicolaidou V 

PROVIDER: S-EPMC5054849 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature