Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

ABSTRACT: Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display translational plasticity to cope with the supply of imported nuclear-encoded subunits. Ribosomes expressing mitochondrial-encoded COX1 mRNA selectively engage with cytochrome c oxidase assembly factors in the inner membrane. Assembly defects of the cytochrome c oxidase arrest mitochondrial translation in a ribosome nascent chain complex with a partially membrane-inserted COX1 translation product. This complex represents a primed state of the translation product that can be retrieved for assembl

SUBMITTER: Richter-Dennerlein R 

PROVIDER: S-EPMC5055049 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature