Microarray screening of Guillain-Barre syndrome sera for antibodies to glycolipid complexes.

ABSTRACT:

Objective

To characterize the patterns of autoantibodies to glycolipid complexes in a large cohort of Guillain-Barré syndrome (GBS) and control samples collected in Bangladesh using a newly developed microarray technique.

Methods

Twelve commonly studied glycolipids and lipids, plus their 66 possible heteromeric complexes, totaling 78 antigens, were applied to polyvinylidene fluoride-coated slides using a microarray printer. Arrays were probed with 266 GBS and 579 control sera (2 μL per serum, diluted 1/50) and bound immunoglobulin G detected with secondary antibody. Scanned arrays were subjected to statistical analyses.

Results

Measuring antibodies to single targets was 9% less sensitive than to heteromeric complex targets (49.2% vs 58.3%) without significantly affect

SUBMITTER: Halstead SK 

PROVIDER: S-EPMC5055300 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature