Influence of Rotational Nucleosome Positioning on Transcription Start Site Selection in Animal Promoters.

ABSTRACT: The recruitment of RNA-Pol-II to the transcription start site (TSS) is an important step in gene regulation in all organisms. Core promoter elements (CPE) are conserved sequence motifs that guide Pol-II to the TSS by interacting with specific transcription factors (TFs). However, only a minority of animal promoters contains CPEs. It is still unknown how Pol-II selects the TSS in their absence. Here we present a comparative analysis of promoters' sequence composition and chromatin architecture in five eukaryotic model organisms, which shows the presence of common and unique DNA-encoded features used to organize chromatin. Analysis of Pol-II initiation patterns uncovers that, in the absence of certain CPEs, there is a strong correlation between the spread of initiation and the intensity of t

SUBMITTER: Dreos R 

PROVIDER: S-EPMC5055345 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature