Expression and regulation of ATF6α in the mouse uterus during embryo implantation.

ABSTRACT:

Background

ATF6α, one of the sensor proteins in the stress signaling pathway of the endoplasmic reticulum, is located in the membrane of the endoplasmic reticulum. To date, the physiological function of ATF6α in the process of embryo implantation has not been reported.

Methods

In this study, the expression pattern of ATF6α in the mouse uterus during peri-implantation and the estrous cycle was detected by real-time PCR, western blot and immunohistochemistry.

Results

ATF6α mRNA and protein levels were higher in the uterus near the implantation site on day 5 and were intensely expressed in the secondary decidual zone (SDZ) on days 7-8. In the uteri of pseudopregnant mice, ATF6α mRNA and protein levels were lower on day 5 than on other days. The activating blastocyst and

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC5055674 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature