Optimization of culture conditions by response surface methodology and unstructured kinetic modeling for bioactive metabolite production by Nocardiopsis litoralis VSM-8.

ABSTRACT: Response surface methodology-based central composite design on five variables incubation time, pH, temperature, sucrose concentration, and soya peptone concentration was employed for optimization of the production of bioactive compounds by Nocardiopsis litoralis strain VSM 8. The main quadratic effects and interactions of the five variables on the production of bioactive metabolites were investigated. A second-order polynomial model produced a satisfied fit for experimental data with regard to the production of the bioactive metabolites. Regression analysis showed that high R ² values of all the five responses are significant and adjusted R ² values showed good agreement with the experimental and predicted values. The present model was used to evaluate the direct inte

SUBMITTER: Managamuri U 

PROVIDER: S-EPMC5055877 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature