Single-cell dynamics and variability of MAPK activity in a yeast differentiation pathway.

ABSTRACT: In response to pheromones, yeast cells activate a MAPK pathway to direct processes important for mating, including gene induction, cell-cycle arrest, and polarized cell growth. Although a variety of assays have been able to elucidate signaling activities at multiple steps in the pathway, measurements of MAPK activity during the pheromone response have remained elusive, and our understanding of single-cell signaling behavior is incomplete. Using a yeast-optimized FRET-based mammalian Erk-activity reporter to monitor Fus3 and Kss1 activity in live yeast cells, we demonstrate that overall mating MAPK activity exhibits distinct temporal dynamics, rapid reversibility, and a graded dose dependence around the K_D of the receptor, where phenotypic transitions occur. The complex dose resp

SUBMITTER: Conlon P 

PROVIDER: S-EPMC5056058 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature