WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors.

ABSTRACT: Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is vital for cells to salvage these proteins before degradation, but the mechanisms for sorting them are not known. Here we describe the evolutionarily conserved recruitment of the WASH (WASP and SCAR homolog) complex to both macropinosomes and phagosomes within a minute of internalization. Using Dictyostelium, we demonstrate that WASH drives protein sorting and recycling from macropinosomes and is thus essential to maintain surface receptor levels and sustain phagocytosis. WASH functionally interacts with the

SUBMITTER: Buckley CM 

PROVIDER: S-EPMC5056073 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature