Cell cycle progression in Caulobacter requires a nucleoid-associated protein with high AT sequence recognition.

ABSTRACT: Faithful cell cycle progression in the dimorphic bacterium Caulobacter crescentus requires spatiotemporal regulation of gene expression and cell pole differentiation. We discovered an essential DNA-associated protein, GapR, that is required for Caulobacter growth and asymmetric division. GapR interacts with adenine and thymine (AT)-rich chromosomal loci, associates with the promoter regions of cell cycle-regulated genes, and shares hundreds of recognition sites in common with known master regulators of cell cycle-dependent gene expression. GapR target loci are especially enriched in binding sites for the transcription factors GcrA and CtrA and overlap with nearly all of the binding sites for MucR1, a regulator that controls the establishment of swarmer cell fate. Despite constitutive synth

SUBMITTER: Ricci DP 

PROVIDER: S-EPMC5056096 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature