Macrophages modulate the growth and differentiation of rhesus monkey embryonic trophoblasts.

ABSTRACT:

Problem

Immune cells within the endometrium at implantation are thought to play an important role in implantation, although their exact role is not well understood.

Method of study

A co-culture system of rhesus monkey embryos and maternal immune cells was established. Blastocysts obtained by in vitro fertilization were co-cultured with peripheral blood cells or decidual macrophages. Culture media were collected to assess secretions. Embryo growth was monitored, and trophoblasts were evaluated for proliferation, apoptosis, and differentiation.

Results

Embryonic trophoblast outgrowths were visible within 6 days of culture, and the area of embryo outgrowth was reduced when blastocysts were cultured with peripheral-derived or decidual macrophages. Trophoblast proliferatio

SUBMITTER: Rozner AE 

PROVIDER: S-EPMC5056160 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature