Mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone induces vasorelaxation without involving K_ATP channel activation in smooth muscle cells of arteries.

ABSTRACT:

Background and purpose

The effects and mechanisms of chemical mitochondrial uncouplers on vascular function have never been identified. Here, we characterized the effects of the typical mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) on vascular function in rat mesenteric arteries and aorta and elucidated the potential mechanisms.

Experimental approach

Isometric tension of mesenteric artery and thoracic aorta was recorded by using a multiwire myograph system. Protein levels were measured by western blot analyses. Cytosolic [Ca²⁺ ]_i , mitochondrial ROS (mitoROS) and mitochondrial membrane potential of smooth muscle cells (A10) were measured by laser scanning confocal microscopy.

Key results

Acute treatment with C

SUBMITTER: Zhang YQ 

PROVIDER: S-EPMC5056225 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature