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Modulation of the E.?coli rpoH Temperature Sensor with Triptycene-Based Small Molecules.

ABSTRACT: Regulation of the heat shock response (HSR) is essential in all living systems. In E.?coli, the HSR is regulated by an alternative ??factor, ?(32) , which is encoded by the rpoH gene. The mRNA of rpoH adopts a complex secondary structure that is critical for the proper translation of the ?(32) protein. At low temperatures, the rpoH gene transcript forms a highly structured mRNA containing several three-way junctions, including a rare perfectly paired three-way junction (3WJ). This complex secondary structure serves as a primitive but highly effective strategy for the thermal control of gene expression. In this work, the first small-molecule modulators of the E.?coli ?(32) mRNA temperature sensor are reported.

SUBMITTER: Barros SA

PROVIDER: S-EPMC5056428 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Modulation of the E. coli rpoH Temperature Sensor with Triptycene-Based Small Molecules.

Barros Stephanie A SA Yoon Ina I Chenoweth David M DM

Angewandte Chemie (International ed. in English) 20160530 29

Regulation of the heat shock response (HSR) is essential in all living systems. In E. coli, the HSR is regulated by an alternative σ factor, σ(32) , which is encoded by the rpoH gene. The mRNA of rpoH adopts a complex secondary structure that is critical for the proper translation of the σ(32) protein. At low temperatures, the rpoH gene transcript forms a highly structured mRNA containing several three-way junctions, including a rare perfectly paired three-way junction (3WJ). This complex second ...[more]

PMID: 27240201

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