Cellular and Matrix Response of the Mandibular Condylar Cartilage to Botulinum Toxin.

ABSTRACT:

Objectives

To evaluate the cellular and matrix effects of botulinum toxin type A (Botox) on mandibular condylar cartilage (MCC) and subchondral bone.

Materials and methods

Botox (0.3 unit) was injected into the right masseter of 5-week-old transgenic mice (Col10a1-RFPcherry) at day 1. Left side masseter was used as intra-animal control. The following bone labels were intraperitoneally injected: calcein at day 7, alizarin red at day 14 and calcein at day 21. In addition, EdU was injected 48 and 24 hours before sacrifice. Mice were sacrificed 30 days after Botox injection. Experimental and control side mandibles were dissected and examined by x-ray imaging and micro-CT. Subsequently, MCC along with the subchondral bone was sectioned and stained with tartrate resistant acid pho

SUBMITTER: Dutra EH 

PROVIDER: S-EPMC5056741 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature