Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing.

ABSTRACT: The major histocompatibility complex (MHC) region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determines the nature of immune responses. Our current knowledge of the bovine MHCI repertoire is limited with only the Holstein-Friesian breed having been studied in any depth. Traditional methods of MHCI genotyping are of low resolution and laborious and this has been a major impediment to a more comprehensive analysis of the MHCI repertoire of other cattle breeds. Next-generation sequencing (NGS) technologies have been used to enable high throughput and much higher resolution MHCI typing in a number

SUBMITTER: Vasoya D 

PROVIDER: S-EPMC5056950 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature