Differential response of immune-related genes to peptidoglycan and lipoteichoic acid challenge in vitro.

ABSTRACT:

Aim

To study the effect of Staphylococcus aureus cell wall antigens, peptidoglycan (PGN) and lipoteichoic acid (LTA) challenge on immune cells present in bovine peripheral blood mononuclear cells (PBMCs).

Materials and methods

In this study, efforts have been made to investigate the effects of three combinations (10+10, 20+20 and 30+30 μg/ml) of PGN and LTA obtained from S. aureus. These antigens were used to challenge the bovine PBMCs. After 6 h of incubation quantitative, real time-polymerase chain reaction was used to study toll-like receptor 2 (TLR-2) and major cytokine mRNA expression in bovine PBMC challenged with three different antigen blends.

Results

The results indicated that mRNA level of interferon gamma is influenced by the expression of TLR

SUBMITTER: Sulabh S 

PROVIDER: S-EPMC5057038 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature