Glycosylation site occupancy in health, congenital disorder of glycosylation and fatty liver disease.

ABSTRACT: Glycosylation is an integral part in health and disease, as emphasized by the growing number of identified glycosylation defects. In humans, proteins are modified with a diverse range of glycoforms synthesized in complex biosynthetic pathways. Glycosylation disorders have been described in congenital disorders of glycosylation (CDG) as well as in acquired disease conditions such and non-alcoholic fatty liver disease (NAFLD). A hallmark in a subset of CDG cases is the reduced glycosylation site occupancy of asparagine-linked glycans. Using an optimized method protocol, we determined the glycosylation site occupancy from four proteins of hepatic and lymphatic origin from CDG and NAFLD patients. We found variable degrees of site occupancy, depending on the tissue of origin and the disease con

SUBMITTER: Hulsmeier AJ 

PROVIDER: S-EPMC5057071 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature