Short-Lived Cages Restrict Protein Diffusion in the Plasma Membrane.

ABSTRACT: The plasma membrane is a heterogeneous environment characterized by anomalous diffusion and the presence of microdomains that are molecularly distinct from the bulk membrane. Using single particle tracking of the C-type lectin CD93, we have identified for the first time the transient trapping of transmembrane proteins in cage-like microdomains which restrict protein diffusion. These cages are stabilized by actin-dependent confinement regions, but are separate structures with sizes and lifespans uncorrelated to those of the underlying actin corral. These membrane cages require cholesterol for their strength and stability, with cholesterol depletion decreasing both. Despite this, cages are much larger in size and are longer lived than lipid rafts, suggesting instead that cholesterol-dependen

SUBMITTER: Goiko M 

PROVIDER: S-EPMC5057110 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature