Three-dimensional electron microscopy reveals the evolution of glomerular barrier injury.

ABSTRACT: Glomeruli are highly sophisticated filters and glomerular disease is the leading cause of kidney failure. Morphological change in glomerular podocytes and the underlying basement membrane are frequently observed in disease, irrespective of the underlying molecular etiology. Standard electron microscopy techniques have enabled the identification and classification of glomerular diseases based on two-dimensional information, however complex three-dimensional ultrastructural relationships between cells and their extracellular matrix cannot be easily resolved with this approach. We employed serial block face-scanning electron microscopy to investigate Alport syndrome, the commonest monogenic glomerular disease, and compared findings to other genetic mouse models of glomerular disease (Myo1e-/-

SUBMITTER: Randles MJ 

PROVIDER: S-EPMC5057164 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature