Production of an anti-dermatophyte monoclonal antibody and its application: immunochromatographic detection of dermatophytes.

ABSTRACT: Tinea refers to superficial infection with one of three fungal genera-Microsporum, Epidermophyton, or Trichophyton-that are collectively known as dermatophytes. These infections are among the most common diseases worldwide and cause chronic morbidity. They are usually diagnosed by direct microscopy and fungal culture, which are burdensome to perform in the clinical setting. To supplement conventional methods, we developed a new method that employs an immunochromatography test for detection of dermatophyte infections. First, anti-Trichophyton monoclonal antibodies (mAb) were produced in mice using a Trichophyton allergen solution as an immunogen. The mAb specificity was assessed by immunostaining alcohol fixed slide cultures and formalin fixed paraffin-embedded microbial samples. Both alcoh

SUBMITTER: Noriki S 

PROVIDER: S-EPMC5057457 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature