Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction.

ABSTRACT:

Background

Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from essential oils or as a byproduct of orange juice production, however, considering the increasing need for limonene and a certain amount of pesticides may exist in the limonene obtained from the citrus industry, some other methods should be explored to produce limonene.

Results

To construct the limonene synthetic pathway in Yarrowia lipolytica, two genes encoding neryl diphosphate synthase 1 (NDPS1) and limonene synthase (LS) were codon-optimized and heterologously expressed in Y. lipolytica. Furthermore, to maximize limonene production, several ge

SUBMITTER: Cao X 

PROVIDER: S-EPMC5057495 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature