PCDH10 Interacts With hTERT and Negatively Regulates Telomerase Activity.

ABSTRACT: Telomerase catalyzes telomeric DNA synthesis, an essential process to maintain the length of telomere for continuous cell proliferation and genomic stability. Telomerase is activated in gametes, stem cells, and most tumor cells, and its activity is tightly controlled by a catalytic human telomerase reverse transcriptase (hTERT) subunit and a collection of associated proteins. In the present work, normal human testis tissue was used for the first time to identify proteins involved in the telomerase regulation under normal physiological conditions. Immunoprecipitation was performed using total protein lysates from the normal testis tissue and the proteins of interest were identified by microfluidic high-performance liquid chromatography and tandem mass spectrometry (HPLC-Chip-MS/MS). The reg

SUBMITTER: Zhou LN 

PROVIDER: S-EPMC5058908 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature