Attenuated innate immune defenses in very premature neonates during the neonatal period.

ABSTRACT:

Background

Antimicrobial responses have been shown to be profoundly attenuated in very preterm neonates when examined on cord blood. However, we lack data on these responses at the time these neonates are most vulnerable to infections.

Methods

Multiple cytokine responses to two prototypic Toll-like receptor (TLR) agonists: lipopolysaccharide (LPS) (TLR4) and R848 (TLR7/8) were prospectively measured in preterm neonates born ≤30 wk of gestation (n = 50) during the first 28 d of age using whole blood and single-cell multiparameter flow cytometry assays. Results were compared to term neonates (n = 30) and adult controls (n = 25).

Results

In preterm neonates, LPS and R848 responses remained attenuated in both cord blood and in the first 28 d of age. These responses showed

SUBMITTER: Marchant EA 

PROVIDER: S-EPMC5059157 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature