Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu²⁺ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity.

ABSTRACT: The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events. This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu²⁺ between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi). The probe DHC can coordinate with Cu²⁺ and consequently display on-off type fluorescence response. Furthermore, the in situ formed nonfluorescent Cu²⁺-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu²⁺ and PPi than that between Cu²⁺ and DHC. The subsequent

SUBMITTER: Zhao L 

PROVIDER: S-EPMC5059578 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature