A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.

ABSTRACT: Antibodies and B cell receptors often bind their antigen at cell-cell interface while both molecular species are surface-bound, which impacts bond kinetics and function. Despite the description of complex energy landscapes for dissociation kinetics which may also result in significantly different association kinetics, surface-bound molecule (2D) association kinetics usually remain described by an on-rate due to crossing of a single free energy barrier, and few experimental works have measured association kinetics under conditions implying force and two-dimensional relative ligand-receptor motion. We use a new laminar flow chamber to measure 2D bond formation with systematic variation of the distribution of encounter durations between antigen and antibody, in a range from 0.1 to 10 ms. Unde

SUBMITTER: Limozin L 

PROVIDER: S-EPMC5059681 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature