A Novel Computer-Assisted Approach to evaluate Multicellular Tumor Spheroid Invasion Assay.

ABSTRACT: Multicellular tumor spheroids (MCTSs) embedded in a matrix are re-emerging as a powerful alternative to monolayer-based cultures. The primary information gained from a three-dimensional model is the invasiveness of treatment-exposed MCTSs through the acquisition of light microscopy images. The amount and complexity of the acquired data and the bias arisen by their manual analysis are disadvantages calling for an automated, high-throughput analysis. We present a universal algorithm we developed with the scope of being robust enough to handle images of various qualities and various invasion profiles. The novelty and strength of our algorithm lie in: the introduction of a multi-step segmentation flow, where each step is optimized for each specific MCTS area (core, halo, and periphery); the qu

SUBMITTER: Cisneros Castillo LR 

PROVIDER: S-EPMC5059692 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature