Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site.

ABSTRACT:

Background

Proteins from the LytR-CpsA-Psr family are found in almost all Gram-positive bacteria. Although LCP proteins have been studied in other pathogens, their functions in enterococci remain uncharacterized. The Psr protein from Enterococcus hirae, here renamed LcpA, previously associated with the regulation of the expression of the low-affinity PBP5 and β-lactam resistance, has been characterized.

Results

LcpA protein of E. hirae ATCC 9790 has been produced and purified with and without its transmembrane helix. LcpA appears, through different methods, to be localized in the membrane, in agreement with in silico predictions. The interaction of LcpA with E. hirae cell wall indicates that LcpA binds enterococcal peptidoglycan, regardless of the presence of secondary cell

SUBMITTER: Marechal M 

PROVIDER: S-EPMC5059904 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature