Screening for peptides targeted to IL-7Rα for molecular imaging of rheumatoid arthritis synovium.

ABSTRACT:

Background

Interleukin-7 receptor alpha (IL-7Rα) represents a biomarker with potential applications in rheumatoid arthritis (RA) diagnosis and therapy. We have therefore searched by phage display potential IL-7Rα specific peptides with the primary goal being to develop in vivo molecular imaging tools.

Methods

IL-7Rα-targeted peptides were searched within a disulfide-constrained combinatorial phage displayed library of random linear heptapeptides. The apparent dissociation constant (K_d) and half maximal inhibition constant (IC₅₀) were estimated for phage clones and synthesized peptides by ELISA. We used 5-Aza-2'-deoxycytidine (ADC)-stimulated Jurkat cells and human synovial tissue from patients with RA for in vitro characterization of peptides. For molec

SUBMITTER: Burtea C 

PROVIDER: S-EPMC5059943 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature