Phylogenetic analysis of human rhinoviruses collected over four successive years in Sydney, Australia.

ABSTRACT:

Background

Human rhinoviruses (HRV) cause a wide spectrum of disease, ranging from a mild influenza-like illness (ILI) to severe respiratory infection. Molecular epidemiological data are limited for HRV circulating in the Southern Hemisphere.

Objectives

To identify the species and genotypes of HRV from clinical samples collected in Sydney, Australia, from 2006 to 2009.

Methods

Combined nose and throat swabs or nasopharyngeal aspirates collected from individuals with ILI were tested for HRV using real-time reverse-transcriptase polymerase chain reaction (RT-PCR). Sequencing data of 5'UTR and VP4/VP2 coding regions on RT-PCR-positive specimens were analysed.

Results

Human rhinoviruses were detected by real-time PCR in 20.9% (116/555) of samples tested. Phylogenet

SUBMITTER: Ratnamohan VM 

PROVIDER: S-EPMC5059946 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature