Rapid detection and subtyping of European swine influenza viruses in porcine clinical samples by haemagglutinin- and neuraminidase-specific tetra- and triplex real-time RT-PCRs.

ABSTRACT:

Background

A diversifying pool of mammalian-adapted influenza A viruses (IAV) with largely unknown zoonotic potential is maintained in domestic swine populations worldwide. The most recent human influenza pandemic in 2009 was caused by a virus with genes originating from IAV isolated from swine. Swine influenza viruses (SIV) are widespread in European domestic pig populations and evolve dynamically. Knowledge regarding occurrence, spread and evolution of potentially zoonotic SIV in Europe is poorly understood.

Objectives

Efficient SIV surveillance programmes depend on sensitive and specific diagnostic methods which allow for cost-effective large-scale analysis.

Methods

New SIV haemagglutinin (HA) and neuraminidase (NA) subtype- and lineage-specific multiplex real-time

SUBMITTER: Henritzi D 

PROVIDER: S-EPMC5059951 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature