A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A.

ABSTRACT:

Background

Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have not been systematically compared for a large number of influenza viruses.

Methods

A total of 151 study participants from near Guangzhou, China, were enrolled in 2009 and provided serum. HI and NT assays were performed for 12 historic and recently circulating strains of seasonal influenza A. We compared titers using Spearman correlation and fit models to predict NT using HI results.

Results

We observed high positive mean correlation between HI and NT assays (Spearman's rank correlation, ρ=.86) across all strains. Co

SUBMITTER: Truelove S 

PROVIDER: S-EPMC5059953 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature