Near-Infrared Intraoperative Chemiluminescence Imaging.

ABSTRACT: Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near-infrared light upon exposure to an aqueous solution of Ce(4+) in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9?pmol?cm(-2) of the transition-metal-based ICI agent. As a proof of concept, we use ICI for the in?vivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal-to-noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies.

SUBMITTER: Buchel GE 

PROVIDER: S-EPMC5061038 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature