An Open Data Format for Visualization and Analysis of Cross-Linked Mass Spectrometry Results.

ABSTRACT: Protein-protein interactions are an important element in the understanding of protein function, and chemical cross-linking shotgun mass spectrometry is rapidly becoming a routine approach to identify these specific interfaces and topographical interactions. Protein cross-link data analysis is aided by dozens of algorithm choices, but hindered by a lack of a common format for representing results. Consequently, interoperability between algorithms and pipelines utilizing chemical cross-linking remains a challenge. pepXML is an open, widely-used format for representing spectral search algorithm results that has facilitated information exchange and pipeline development for typical shotgun mass spectrometry analyses. We describe an extension of this format to incorporate cross-linking spectral

SUBMITTER: Hoopmann MR 

PROVIDER: S-EPMC5061604 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature