Transitioning from Targeted to Comprehensive Mass Spectrometry Using Genetic Algorithms.

ABSTRACT: Targeted proteomic assays are becoming increasingly popular because of their robust quantitative applications enabled by internal standardization, and they can be routinely executed on high performance mass spectrometry instrumentation. However, these assays are typically limited to 100s of analytes per experiment. Considerable time and effort are often expended in obtaining and preparing samples prior to targeted analyses. It would be highly desirable to detect and quantify 1000s of analytes in such samples using comprehensive mass spectrometry techniques (e.g., SWATH and DIA) while retaining a high degree of quantitative rigor for analytes with matched internal standards. Experimentally, it is facile to port a targeted assay to a comprehensive data acquisition technique. However, data an

SUBMITTER: Jaffe JD 

PROVIDER: S-EPMC5061621 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature