Exploring the mechanisms of action of human secretory RNase 3 and RNase 7 against Candida albicans.

ABSTRACT: Human antimicrobial RNases, which belong to the vertebrate RNase A superfamily and are secreted upon infection, display a wide spectrum of antipathogen activities. In this work, we examined the antifungal activity of the eosinophil RNase 3 and the skin-derived RNase 7, two proteins expressed by innate cell types that are directly involved in the host defense against fungal infection. Candida albicans has been selected as a suitable working model for testing RNase activities toward a eukaryotic pathogen. We explored the distinct levels of action of both RNases on yeast by combining cell viability and membrane model assays together with protein labeling and confocal microscopy. Site-directed mutagenesis was applied to ablate either the protein active site or the key anchoring region for cell

SUBMITTER: Salazar VA 

PROVIDER: S-EPMC5061719 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature