Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments.

ABSTRACT: When deprived of FtsZ, Escherichia coli cells (VIP205) grown in liquid form long nonseptated filaments due to their inability to assemble an FtsZ ring and their failure to recruit subsequent divisome components. These filaments fail to produce colonies on solid medium, in which synthesis of FtsZ is induced, upon being diluted by a factor greater than 4. However, once the initial FtsZ levels are recovered in liquid culture, they resume division, and their plating efficiency returns to normal. The potential septation sites generated in the FtsZ-deprived filaments are not annihilated, and once sufficient FtsZ is accumulated, they all become active and divide to produce cells of normal length. FtsZ-deprived cells accumulate defects in their physiology, including an abnormally high number of un

SUBMITTER: Sanchez-Gorostiaga A 

PROVIDER: S-EPMC5061873 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature