Virome characterisation from Guthrie cards in children who later developed acute lymphoblastic leukaemia.

ABSTRACT:

Background

Some childhood acute lymphoblastic leukaemias (ALL) can be traced back to a prenatal origin, where a virus infection could be involved in the first pre-leukaemic clone development. The DNA virome of 95 children who later developed ALL was characterised from neonatal blood spots (NBS) using unbiased next-generation sequencing (NGS) and compared with the virome of 95 non-ALL controls.

Methods

DNA was individually extracted from the ALL-patients and controls, pooled, randomly amplified and sequenced using the Illumina MiSeq Sequencing System.

Results

Virus-like sequences identified in both groups mapped to human endogenous retroviruses and propionibacterium phage, considered a part of the normal microbial flora. Potential pathogens human herpesvirus type 6 (HH

SUBMITTER: Bogdanovic G 

PROVIDER: S-EPMC5061901 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature