High-resolution crystal structure of the therapeutic antibody pembrolizumab bound to the human PD-1.

ABSTRACT: Pembrolizumab is an FDA-approved therapeutic antibody that targets the programmed cell death-1 (PD-1) to block the immune checkpoint pathway for the treatment of various types of cancer. It receives remarkable attention due to the high degree of efficacy. Very recently, the crystal structure of the Fab fragment of pembrolizumab (PemFab) in complex with the extracellular domain of human PD-1 (PD-1_ECD) was reported at a resolution of 2.9 Å. However, this relatively low-resolution structural data fails to provide sufficient information on interfacial water molecules at the binding interface that substantially contribute to affinity and specificity between the therapeutic antibody and target. Here, we present the independently determined crystal structure of the Fv fragment of pembr

SUBMITTER: Horita S 

PROVIDER: S-EPMC5062252 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature