Actomyosin-dependent dynamic spatial patterns of cytoskeletal components drive mesoscale podosome organization.

ABSTRACT: Podosomes are cytoskeletal structures crucial for cell protrusion and matrix remodelling in osteoclasts, activated endothelial cells, macrophages and dendritic cells. In these cells, hundreds of podosomes are spatially organized in diversely shaped clusters. Although we and others established individual podosomes as micron-sized mechanosensing protrusive units, the exact scope and spatiotemporal organization of podosome clustering remain elusive. By integrating a newly developed extension of Spatiotemporal Image Correlation Spectroscopy with novel image analysis, we demonstrate that F-actin, vinculin and talin exhibit directional and correlated flow patterns throughout podosome clusters. Pattern formation and magnitude depend on the cluster actomyosin machinery. Indeed, nanoscopy reveals m

SUBMITTER: Meddens MB 

PROVIDER: S-EPMC5062568 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature