Systemic AAV9-IFNβ gene delivery treats highly invasive glioblastoma.

ABSTRACT:

Background

Complete surgical removal of all glioblastoma (GBM) cells is impossible due to extensive infiltration into brain parenchyma that ultimately leads to tumor recurrence. The current standard of care affords modest improvements in survival. New therapeutic interventions are needed to prevent recurrence. Local AAV-hIFNβ gene delivery to the brain was previously shown to eradicate noninvasive orthotopic U87 tumors in mice. However, widespread CNS gene delivery may be necessary to treat invasive GBMs. Here we investigated the therapeutic effectiveness of systemically infused AAV9-hIFNβ against an invasive orthotopic GBM8 model.

Methods

Mice bearing human GBM8 brain tumors expressing firefly luciferase (Fluc) were treated systemically with different doses of scAAV9-hIFNβ

SUBMITTER: GuhaSarkar D 

PROVIDER: S-EPMC5063516 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature