Study of the degradation of a multidrug transporter using a non-radioactive pulse chase method.

ABSTRACT: Proteins are constantly synthesized and degraded in living cells during their growth and division, often in response to metabolic and environmental conditions. The synthesis and breakdown of proteins under different conditions reveal information about their mechanism of function. The metabolic incorporation of non-natural amino acid azidohomoalanine (AHA) and subsequent labeling via click chemistry emerged as a non-radioactive strategy useful in the determination of protein kinetics and turnover. We used the method to monitor the degradation of two proteins involved in the multidrug efflux in Escherichia coli, the inner membrane transporter AcrB and its functional partner membrane fusion protein AcrA. Together they form a functional complex with an outer membrane channel TolC to actively t

SUBMITTER: Chai Q 

PROVIDER: S-EPMC5063704 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature