Proteome data from a host-pathogen interaction study with Staphylococcus aureus and human lung epithelial cells.

ABSTRACT: To simultaneously obtain proteome data of host and pathogen from an internalization experiment, human alveolar epithelial A549 cells were infected with Staphylococcus aureus HG001 which carried a plasmid (pMV158GFP) encoding a continuously expressed green fluorescent protein (GFP). Samples were taken hourly between 1.5 h and 6.5 h post infection. By fluorescence activated cell sorting GFP-expressing bacteria could be enriched from host cell debris, but also infected host cells could be separated from those which did not carry bacteria after contact (exposed). Additionally, proteome data of A549 cells which were not exposed to S. aureus but underwent the same sample processing steps are provided as a control. Time-resolved changes in bacterial protein abundance were quantified

SUBMITTER: Surmann K 

PROVIDER: S-EPMC5063755 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature