Identification of SNPs in a nonmodel macrofungus (Lepista nuda, Basidiomycota) through RAD sequencing.

ABSTRACT: Lepista nuda is a wild edible fungus that is valued for its odor and taste. Recent studies identified intraspecific morphological and genetic differences in L. nuda. Although single-nucleotide polymorphisms (SNPs) are useful for revealing intraspecific differences, the traditional methods used for investigating SNPs are time consuming and expensive, and they only locate a limited number of SNPs. This study used a "restriction-site associated DNA" (RAD) method combined with high throughput sequencing to efficiently identify a large number of SNPs in two samples of L. nuda. A total of 7 and 9 billion bp of raw data were obtained from the two collections. A total of 712 SNPs were found. These SNPs will be useful for the further analysis of the genetic variation within

SUBMITTER: Ye F 

PROVIDER: S-EPMC5063826 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature