Ratiometric ultrasensitive electrochemical immunosensor based on redox substrate and immunoprobe.

ABSTRACT: In this work, we presented a ratiometric electrochemical immunosensor based on redox substrate and immunoprobe. Carboxymethyl cellulose-Au-Pb²⁺ (CMC-Au-Pb²⁺) and carbon-Au-Cu²⁺ (C-Au-Cu²⁺) nanocomposites were firstly synthesized and implemented as redox substrate and immunoprobe with strong current signals at -0.45 V and 0.15 V, respectively. Human immunoglobulin G (IgG) was used as a model analyte to examine the analytical performance of the proposed method. The current signals of CMC-Au-Pb²⁺ (I_substrate) and C-Au-Cu²⁺ (I_probe) were monitored. The effect of redox substrate and immunoprobe behaved as a better linear relationship between I_probe/I_substrate and Lg C_IgG (ng

SUBMITTER: Tang Z 

PROVIDER: S-EPMC5064308 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature