In vivo dendritic cell targeting cellular vaccine induces CD4⁺ Tfh cell-dependent antibody against influenza virus.

ABSTRACT: An induction of long-term cellular and humoral immunity is for the goal of vaccines, but the combination of antigens and adjuvant remain unclear. Here, we show, using a cellular vaccine carrying foreign protein antigen plus iNKT cell glycolipid antigen, designated as artificial adjuvant vector cells (aAVCs), that mature XCR1^- DCs in situ elicit not only ordinal antigen-specific CD4⁺T cells, but also CD4⁺ Tfh and germinal center, resulted in inducing long-term antibody production. As a mechanism for leading the long-term antibody production by aAVC, memory CD4⁺ Tfh cells but not iNKTfh cells played an important role in a Bcl6 dependent manner. To develop it for influenza infection, we established influenza hemagglutinin-carrying aAVC (aAVC-HA) and

SUBMITTER: Yamasaki S 

PROVIDER: S-EPMC5064395 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature