The effects of hypoxia on the stemness properties of human dental pulp stem cells (DPSCs).

ABSTRACT: Recent studies have demonstrated that culture under hypoxia has beneficial effects on mesenchymal stem cells (MSCs). However, there are limitations to achieving a stable condition in conventional hypoxic CO₂ incubators. DPSCs are a unique type of MSCs which are promising in many regenerative therapies. In this study, we investigated the ideal hypoxic culture environment for DPSCs using a new system that can provide controlled O₂ environment. The effects of hypoxia (3%, 5%) on the stemness properties of DPSCs. Their morphology, proliferation rate, expression of stem cell markers, migration ability, mRNA expression of angiogenic/neurotrophic factors and immunomodulatory genes were evaluated and compared. Additionally, the effect of the discrete secretome on proliferatio

SUBMITTER: Ahmed NE 

PROVIDER: S-EPMC5064411 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature