The interaction between fibrinogen and zymogen FXIII-A2B2 is mediated by fibrinogen residues γ390-396 and the FXIII-B subunits.

ABSTRACT: Coagulation transglutaminase factor XIII (FXIII) exists in circulation as heterotetrameric proenzyme FXIII-A₂B₂ Effectively all FXIII-A₂B₂ circulates bound to fibrinogen, and excess FXIII-B₂ circulates in plasma. The motifs that mediate interaction of FXIII-A₂B₂ with fibrinogen have been elusive. We recently detected reduced binding of FXIII-A₂B₂ to murine fibrinogen that has γ-chain residues 390-396 mutated to alanines (Fibγ^390-396A). Here, we evaluated binding features using human components, including recombinant fibrinogen variants, FXIII-A₂B₂, and isolated FXIII-A₂ and -B₂ homodimers. FXIII-A₂B₂ coprecipitated w

SUBMITTER: Byrnes JR 

PROVIDER: S-EPMC5064719 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature