Saturation monitoring of VX15/2503, a novel semaphorin 4D-specific antibody, in clinical trials.

ABSTRACT:

Background

Receptor occupancy, or saturation, assays are often utilized in preclinical and clinical development programs to evaluate the binding of a biologic to a cellular target. These assays provide critical information regarding the dose of drug required to "saturate" the target as well as important pharmacodymamic (PD) data. A flow cytometric method was developed to measure the degree of Semaphorin 4D (SEMA4D; CD100) saturation by VX15/2303, an investigational monoclonal antibody specific for SEMA4D.

Methods

The assay detects VX15/2503, a human IgG4 specific for SEMA4D, with an IgG4 -specific monoclonal antibody.

Results

Data generated allowed assessment of two related SEMA4D-specific pharmacodynamic (PD) markers: (1) The measurement of cellular SEMA4D (cSEMA4D)

SUBMITTER: Fisher TL 

PROVIDER: S-EPMC5064733 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature