Lack of effects of ooplasm transfer on early development of interspecies somatic cell nuclear transfer bison embryos.

ABSTRACT:

Background

Successful development of iSCNT (interspecies somatic cell nuclear transfer) embryos depends on complex interactions between ooplasmic and nuclear components, which can be compromised by genetic divergence. Transfer of ooplasm matching the genetic background of the somatic cell in iSCNT embryos is a valuable tool to study the degree of incompatibilities between nuclear and ooplasmic components. This study investigated the effects of ooplasm transfer (OT) on cattle (Bos taurus) and plains bison (Bison bison bison) embryos produced by iSCNT and supplemented with or without ooplasm from cattle or plains bison oocytes.

Results

Embryos in all groups were analysed for developmental competence that included cleavage rates, ATP content, and expression of nuclear- and mito

SUBMITTER: Gonzalez-Grajales LA 

PROVIDER: S-EPMC5064788 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature