Effect of propofol on microRNA expression in rat primary embryonic neural stem cells.

ABSTRACT:

Background

Propofol is a widely used intravenous anesthetic that is well-known for its protective effect in various human and animal disease models. However, the effects of propofol on neurogenesis, especially on the development of neural stem cells (NSCs), remains unknown. Related microRNAs may act as important regulators in this process.

Methods

Published Gene Expression Omnibus (GEO) DataSets related to propofol were selected and re-analyzed to screen neural development-related genes and predict microRNA (miRNA) expression using bioinformatic methods. Screening of the genes and miRNAs was then validated by qRT-PCR analysis of propofol-treated primary embryonic NSCs.

Results

Four differentially expressed mRNAs were identified in the screen and 19 miRNAs were predict

SUBMITTER: Fan J 

PROVIDER: S-EPMC5064799 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature